cfaed Seminar Series

Abstract

Recent progress in bioscience and biotechnologies has given us new biological resources for creating novel strategies in wide variety of bioengineering, from biological production to theranostics and tissue engineering. To utilize such biological resources safely and steadily, molecular tools for controlling them in a designed manner are needed. We have created unique chemical tools for controlling such biological targets. Our chemical tools were designed to work at the interfaces of the targets for regulating their interaction effectively. First, a photo-responsive cell immobilization tool could attach living cells at the desired timing and location on the substrate through light-navigated anchoring onto cell surfaces, and dynamically release cells through photo-cleavage reaction. On the photo-responsive substrate, a single cell array was simply prepared and applied to high-throughput single-cell analysis. Second, a photo-cleavable sterically-bulky protection tool could achieve light-induced activation of biopolymers such as proteins and DNA on living cells. This protection tool was applied to photolytic protein aggregates for light-induced protein delivery. 

References

1) Yamaguchi et al, Angew. Chem. Int. Ed., 51, 128-131, 2012. 2) Yamahira et al, Macromol. Biosci., 14, 1670-1676, 2014. 3) Yamaguchi et al, Chem. Commun., 46, 2244-2246, 2010. 4) Takamori et al, Chem. Commun., 49, 3013-3015, 2013. 5) Ishiwatari et al, Adv. Health. Mater., 5, 1002-1007, 2016.